Additive effects of advanced temperature and photoperiod regimes and LHRHa injection on ovulation in Atlantic salmon (Salmo salar)

Abstract

In order to evaluate manipulation of spawning time as a potential means to extend 0+ Atlantic salmon (Salmo salar) smolt production in Tasmania, Australia, female salmon were exposed to a natural/simulated natural (42°S) photoperiod or an advanced (L:D 9:15) photoperiod from the austral summer solstice (20 December) under natural or advanced (~6 °C below natural temperature) temperature conditions. In late summer (26 February) injections of a commercial LHRHa preparation or vehicle (propylene glycol) commenced. Regular ovulation checks were conducted and ova were fertilised using milt from LHRHa-injected males held under matching photo-thermal conditions. Plasma levels of 17β-estradiol (E2) and testosterone (T) were monitored and reproductive success (cumulative % ovulation, % fertilisation and % survival to the eyed-egg stage) was recorded. Ovulations commenced first (09 March) in LHRHa-treated fish that experienced advanced photoperiod and thermal regimes whereas sham-treated fish exposed to natural photoperiod and temperature conditions where the last to ovulate (22 May–08 June). Treatment-related sequential changes in the timing of ovulations were reflected by sequential advances in the timing of peaks in plasma levels of E2 and T. The fertilisation of ova from LHRHa-treated fish that experienced advanced photoperiod and thermal regimes was significantly reduced (~52%) relative to all other treatments (>80%) but there were no significant treatment-related differences in the survivals of eggs to the eyed stage (~50–90%). Consequently, a maximum advance in the timing of median ovulation of 71 days and commercially acceptable eyed-egg yields were generated, demonstrating that combinations of photoperiod, thermal and hormone treatments may be employed to significantly extend spawning and thereafter increase the availability of 0+ smolts for grow-out.