Additive and dose-dependent mixture effects of Flumite 200 (flufenzin, acaricide) and Quadris (azoxystrobin, fungicide) on the reproduction and survival of Folsomia candida (Collembola)

Abstract

Chemical plant protection is still a dominant agricultural practice in the world, and usually fields are treated with several pesticides many times per year. This means that not only the single substances affect the environment and the non-target organisms, but their mixtures. Our model organism was Folsomia candida (Collembola). We aimed to gain information on the toxicity of Quadris (azoxystrobin) and Flumite 200 (flufenzine aka. diflovidazine) on survival and reproduction and whether the animals can mitigate the toxicity with soil and/or food avoidance behaviour. Also, we aimed to test the effect of the mixture of these two pesticides. We used the OECD 232 reproduction test, a soil avoidance test, and a food choice test for both single pesticides and their mixture. We prepared the mixtures based on the concentration addition model, so the 50% effective concentrations (EC50) of the single materials were used as one toxic unit with a constant ratio of the two materials in the mixture. In the end, the measured mixture EC and LC (lethal concentration) values were compared to the estimate of the concentration addition model. Both materials were toxic to the Collembola in much higher concentrations than the recommended field concentrations (Flumite 200 EC50: 1.096, LC50: 1.561, Quadris EC50: 65.568, LC50: 386.165 mg kg−1). The springtails did not consistently avoid the polluted soils, this only happened in higher concentrations. The mixtures seemed to have additive effects on the reproduction and we found dose-dependent interaction with the survival (EC50: 1.022 Toxic Unit, 0.560 Flumite 200 and 33.505 Quadris; LC50: 1.509 Toxic Unit, 0.827 Flumite 200 and 49.471 mg kg−1 Quadris). The deviation from the concentration addition model suggests that the curve starts with a synergy. but above EC50 it becomes antagonistic. We conclude that both Quadris and Flumite 200 are safe for springtails until the recommended field concentration is respected. However, if higher concentrations are used the animals cannot avoid Flumite 200 and the toxic effects can fully manifest. Consequently, the dose-dependent deviation from the concentration addition model is a reason for caution as the low concentrations were synergistic for survival. That means the field concentrations can possibly cause synergistic effects. However, to clarify that further tests are necessary.