Abstract
Seventeen additional minor ecdysteroid compounds were isolated and identified from the roots of Leuzea carthamoides (Wild.) DC. Eight of them are new phytoecdysteroids: carthamoleusterone ( 13) is a new side-chain cyclo-ether with five-membered ring; 14-epi-ponasterone A 22-glucoside ( 12) is a rare and unusual natural 14β-OH epimer; 15-hydroxyponasterone A ( 11) is also new and rare with its C-15 substituted position, as well as 22-deoxy-28-hydroxymakisterone C ( 18) possessing secondary hydroxyl in position C-28 and 26-hydroxymakisterone C ( 20) with hydroxy groups in positions 25 and 26. New are also 1β-hydroxymakisterone C ( 21) and 20,22-acetonides of inokosterone ( 8) and integristerone A ( 10). Series of already known ecdysteroids: ecdysone ( 1), 20-hydroxyecdysone 2- and 3-acetates ( 3 and 4), turkesterone ( 6), inokosterone ( 7), 24-epi-makisterone A ( 14), and amarasterone A ( 22) are reported here as new constituents of L. carthamoides. Seven earlier reported Leuzea ecdysteroids: 20-hydroxyecdysone ( 2), ajugasterone C ( 5), integristerone A ( 9), 24(28)-dehydromakisterone A ( 15), 24(28)-dehydroamarasterone B ( 16), (24 Z)-29-hydroxy-24(28)-dehydromakisterone C ( 17) and makisterone C ( 19) are also included because they are now better characterized.
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