Abstract
Flow cytometric analysis of forward (cell size) and side (cellular granularity) scatter intensity as an alternative readout for senescence. Approximately 5 % of the cells of the untreated controls were placed in the F/S-high compartment and used as normalization standard as described in the material and methods section. Forward and side scatter analysis by flow cytometry (without reseeding) at the indicated time points during the treatment and recovery phase. Shown are representative images of three experiments. (PPTX 389 kb)
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