Additional file 2: Figure S2. of Nuclear envelope structural proteins facilitate nuclear shape changes accompanying embryonic differentiation and fidelity of gene expression

Abstract

Indirect immunofluorescence detection of lamin A and emerin in ES cells. ES cells were incubated with 1 μM RA for 4 days to induce endoderm differentiation. Cells were fixed and processed for immunofluorescence as described in “Methods.” Primary antibodies used were rabbit polyclonal anti-lamin A (Abcam #26300) and goat polyclonal anti-emerin (Santa Cruz #sc-8086). Secondary antibodies used were Alexa-555-conjugated donkey anti-rabbit and Alexa-488-conjugated donkey anti-mouse antibodies (Molecular Probes, Thermo Fisher Scientific). Nuclei were counterstained with DAPI. Merged images are shown. In the WT sample, for clarity, the box inset shows only immunofluorescence staining for emerin. These images confirm the absence of expression of lamin A and emerin in the mutant ES cells and the low expression of lamin A/C in the emd −/− cells. (PPTX 487 kb)