Abstract

The amino acid sequence alignment and conserved motifs of G-type PtLecRLKs. (A) Amino acid sequence alignment via CLUSTALW. To determine conserved amino acid regions, 50Â % sequence identity was used as a cutoff. Note that the amino acid sequence in the protein kinase domain is highly conserved in most G-type PtLecRLKs whereas the other domains such as S-locus glycoprotein, EGF, PAN, TM and signal peptide varied. Highly conserved motifs are boxed with labels a, b, c, d and e. The red arrows indicate truncated bulb lectin domains. The blue arrows indicate the truncated protein kinase domains. (B) The conserved motif sequences marked on panel A. Sequence logo was generated from the consensus amino acid sequence over 50Â % sequence identity. (PPTX 3107 kb)

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