Abstract
The objective ofthis study isto evaluate the changes inthe motility and kinetic patterns ofcanine spermatozoa, capacitated and decapacitated, after the addition ofseminal plasma protein fractions with different molecular weight. Ithas been proposed that proteins inseminal plasma support the survival ofthe spermatozoa and exert adual effect: capacitation and decapacitation. The seminal plasma from fresh ejaculates was subjected tochromatographic separation and four protein fractions were obtained. Computer-assisted sperm analysis was used todetermine the sperm subpopulations with specific motion and kinetic characteristics after incubation with each ofthe four protein fractions. Two-dimensional electrophoresis ofthe fractions that exhibit asignificant effect onthecapacitation and decapacitation was performed. Bysperm class analyser, capacitation changes were observed inthe sperm subpopulation with ahigh curvilinear velocity and amplitude oflateral head displacement incubatedwith the seminal plasma protein fraction with ahigh molecular weight, which was also reflected inthedecreased linearity, straightness, and progressive motility. The sperm subpopulation incubated with theseminal plasma protein fraction with alow molecular weight seemed toundergo aprocess ofdecapacitation (decreasing ofthe curvilinear velocity, increasing ofthe linearity, straightness and showing progressive motility). Despite their ample panorama ofactions, the role ofseminal plasma proteins regarding capacitation and decapacitation isstill undetermined.
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