Abstract

This study aimed to evaluate the usefulness of bioactive extracts obtained from red wine by-products, such as grape skins and stems, for reducing or eliminating the use of SO2 in red wine production. Special attention was focused on guaranteeing the microbiological stability of the red wines and protecting them against oxidation. Therefore, the antioxidant and antimicrobial activities of the extracts and red wines were studied. Red grape stems and skins, by-products of the wine industry, from six types of monovarietal wines, were used. Extracts obtained from stems displayed higher concentrations of total phenolic compounds and higher in vitro antioxidant activity. Both stem and skin extracts demonstrated higher antimicrobial activity against pathogenic bacteria and lower activity against yeasts. In the wines produced, higher antimicrobial and antioxidant activities were observed, mainly in the skin extract batches. This study highlights that bioactive extracts obtained from by-products of wine making could be used to reduce or eliminate the use of SO2 in wine production. In this way, healthier red wines could be obtained while guaranteeing their microbiological stability and protecting them from oxidation. Furthermore, the use of these by-products is strongly associated with the circular economy, as they could help to reduce the environmental impact of the wine industry.

Highlights

  • Sulfur dioxide (SO2 ) is the most used preservative along the wine production processing chain, from pressing to bottling [1]

  • When the antioxidant activity of stem and grape-skin extracts was evaluated using two methods (DPPH and ABTS), both methods higher antioxidant activity was found in the stem extracts (421.75 and 821.76 mg Trolox/100 g, respectively) than in the skin extracts

  • A higher total phenolic content (TPC) was determined when compared with the grape-skin extracts, which most probably explains the higher antioxidant activity observed in the former

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Summary

Introduction

Sulfur dioxide (SO2 ) is the most used preservative along the wine production processing chain, from pressing to bottling [1] This compound is well known for its antimicrobial properties and antioxidant activity against non-enzymatic oxidation of wines. It functions as an antioxidant to inhibit oxidase enzymes typical of grapes, such as tyrosinase or laccase, in a lower range [2,3] The application of this compound is especially relevant in the production of white wines to avoid losses of sensorial quality due to oxidation reactions. In this sense, it has a great influence on sensorial characteristics, preventing browning and loss of color and aroma complexity [2]. It helps to control the presence of undesirable microorganisms, such as non-Saccharomyces yeast or acetic or lactic acid bacteria [2,4,5]

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