ADDITION OF Caulerpa racemosa EXTRACT ON RPS AND CLINICAL SIGNS OF VANAME SHRIMP AFTER Vibrio parahaemolyticus INFECTION

Abstract

Prevention of AHPND in shrimp can be done by giving immunostimulants, one of which comes fromseaweed extract, explicitly Caulerpa racemosa. This study aimed to determine the effect of the administration of Caulerpa racemosa extract on the relative percent survival (RPS), clinical signs, and behavioral changes of white shrimp after being infected with Vibrio parahaemolyticus. This study used a completely random design with five treatments and three replications, namely K-, K+, and three treatments with the addition of Caulerpa racemosa extract, names P1 (3 μg/g), P2 (6 μg /g), and P3 (9 μg/g). The infection process with Vibrio parahaemolyticus bacteria was carried out 24 hours after being injected with Caulerpa racemosa extract. The results of this study indicate that the highest Relative Percent Survival (RPS) value was P3. Clinical signs of shrimp infected with Vibrio parahaemolyticus were characterized by empty intestines and a whitened hepatopancreas. In addition, there were behavioral changes in shrimp infected with Vibrio parahaemolyticus, namely decreased appetite, passive shrimp movement at the bottom of the aquarium, and erratic swimming movement of shrimp. Based on these results, it could be said that the best treatment is in P3 with a dose of 9 μg/g. This study can provide the best quantity of C. racemosa extract to treat AHPND in shrimp.