Abstract
Burkholderia pyrrocinia JK-SH007 is an important biocontrol strain for the prevention and treatment of poplar canker disease. Its powerful biocontrol function is inseparable from its successful colonization of poplar trees. Bacterial biofilms can ensure the long-term colonization of a host. To explore the mechanism of action of biofilms in the biocontrol process, we manipulated various exogenous factors to explore the morphology of the JK-SH007 biofilm in vitro. The addition of glycerol and MgSO4 to TSB medium stimulated biofilm production, increased the resistance of JK-SH007 to disease, enhanced the survival of JK-SH007 in nutrient-poor environments and maintained the antagonistic ability of JK-SH007 against the poplar canker pathogen. Therefore, we constructed and optimized a biofilm-forming system to produce a large number of stable JK-SH007 biofilms. The optimized system showed that the optimal incubation time for JK-SH007 biofilm formation was 14 h, the optimal temperature of the static culture was 25 °C, and the optimal pH was 5. The optimal medium for biofilm formation was TSB medium, 1% glycerol and 50 mM MgSO4. RT-qPCR experiments showed that an increase in the expression of the suhB gene promoted JK-SH007 biofilm formation, while an increase in the expression level of the ropN gene inhibited JK-SH007 biofilm formation. The possible mechanism by which JK-SH007 was inhibited by biofilm formation under natural culture was revealed. These results indicate the importance of adding nutrients to JK-SH007 biocides produced on a commercial scale. This is the first report of JK-SH007 producing a long-lasting biofilm that guarantees antagonism.
Highlights
Bacterial biofilms are organized and distinct threedimensional structures of life groups (Mah et al 2003)
The biofilm-forming ability of the test medium system based on LB medium was generally weak, and under the same exogenous substance stress, the amount of biofilm formation was lower in LB than in TSB medium
The biofilm morphology of JK-SH007 was observed for the first time using the exogenous stress method
Summary
Bacterial biofilms are organized and distinct threedimensional structures of life groups (Mah et al 2003). Most bacteria in nature exist in biofilms (Whitchurch et al 2002). There is evidence that the formation of a biofilm is an important step in the sustainable development and spread of bacteria (Guilhen 2017), including the exchange of intermediate genes and their. In the process of colonizing host plants and bacteria, bacteria can form biofilms on their surfaces. These biofilms are described as microcolonies, aggregates, cell clusters, etc. Biocontrol bacteria have a significant promoting effect on colonization in the environment surrounding plants. The formation of biofilms greatly promotes the colonization of Bacillus subtilis in Arabidopsis roots and protects Arabidopsis roots from pathogens, such as
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
