Abstract
We have developed an adaptive optics photoacoustic microscope (AO-PAM) for high-resolution imaging of biological tissues, especially the retina. To demonstrate the feasibility of AO-PAM we first designed the AO system to correct the wavefront errors of the illuminating light of PAM. The aberrations of the optical system delivering the illuminating light to the sample in PAM was corrected with a close-loop AO system consisting of a 141-element MEMS-based deformable mirror (DM) and a Shack-Hartmann (SH) wavefront sensor operating at 15 Hz. The photoacoustic signal induced by the illuminating laser beam was detected by a custom-built needle ultrasonic transducer. When the wavefront errors were corrected by the AO system, the lateral resolution of PAM was measured to be better than 2.5 µm using a low NA objective lens. We tested the system on imaging ex vivo ocular samples, e.g., the ciliary body and retinal pigment epithelium (RPE) of a pig eye. The AO-PAM images showed significant quality improvement. For the first time we were able to resolve single RPE cells with PAM.
Highlights
Photoacoustic microscopy (PAM) [1–6] is a novel microscopic imaging modality that can provide three–dimensional, high-resolution vasculature imaging
We have developed an adaptive optics photoacoustic microscope (AO-PAM) for high-resolution imaging of biological tissues, especially the retina
To demonstrate the feasibility of AO-PAM we first designed the AO system to correct the wavefront errors of the illuminating light of PAM
Summary
Photoacoustic microscopy (PAM) [1–6] is a novel microscopic imaging modality that can provide three–dimensional, high-resolution vasculature imaging. It can provide functional imaging of biological tissues such as the blood vessel oxygen saturation by using multiple wavelength illumination. PAM detects the ultrasonic waves (PA waves) generated by pulsed laser-induced localized thermal expansion in biological tissues as a result of specific optical absorption. We developed the optical coherence tomography (OCT) guided photoacoustic ophthalmoscopy (PAOM) [7], which extended the laser scanning optical-. We have successfully used PAOM to image the retinal vasculature and the melanin distribution in the retinal pigment epithelium (RPE) of rat eye in vivo. Imaging RPE is important since its dysfunction contributes to agerelated macular degeneration (AMD), a leading blinding disease in the industrialized world
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
