Abstract
Small animal models of retinal diseases are important to vision research, and noninvasive high resolution in vivo rodent retinal imaging is becoming an increasingly important tool used in this field. We present a custom Fourier domain optical coherence tomography (FD-OCT) instrument for high resolution imaging of mouse retina. In order to overcome aberrations in the mouse eye, we incorporated a commercial adaptive optics system into the sample arm of the refractive FD-OCT system. Additionally, a commercially available refraction canceling lens was used to reduce lower order aberrations and specular back-reflection from the cornea. Performance of the adaptive optics (AO) system for correcting residual wavefront aberration in the mice eyes is presented. Results of AO FD-OCT images of mouse retina acquired in vivo with and without AO correction are shown as well.
Highlights
Small animal models of human diseases serve as a vital component in modern vision research
A custom adaptive optics (AO) Fourier domain optical coherence tomography (FD-OCT) system built at Simon Frasier University was used for mouse retinal imaging
Since the FD-OCT and the wavefront sensor (WFS) used the same light source, the effects to the WFS spots could be monitored as the location of the focal plane on the mouse retina was adjusted by looking at B-scan
Summary
Small animal models of human diseases serve as a vital component in modern vision research Rodents, such as mice and rats, are commonly used in the development of novel therapeutics against diseases causing blindness. To better visualize the cellular microstructure and understand the molecular processes in the living retina, high resolution retinal imaging of rodent eyes is desirable. Techniques such as immunohistology provide a gold standard for studying the animal model retinas with exquisite resolution and a variety of molecular contrast stains, but the invasiveness of the procedure limits it to a single time point and implies the use of many animals. The cone packing density closer to the fovea, or rod photoreceptor packing, is much higher, so a large pupil size and AO correction are required to increase the resolution and allow visualization of these structures
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