Abstract
We report on a 3D printed microscope, based on a design by the Openflexure project, that uses low cost components to perform fluorescence imaging. The system is sufficiently sensitive and mechanically stable to allow the use of the Super Resolution Radial Fluctuations algorithm to obtain images with resolution better than the diffraction limit. Due to the low-cost components, the entire system can be built for approximately $1200.
Highlights
The Openflexure microscope is an open-access, 3D printable microscope which has three dimensional movement of the specimen stage provided by a flexible plastic mechanism[1]
Super-resolution radial fluctuations (SRRF) allows super resolution information to be extracted from a series of fluorescence images taken with a high numerical-aperture (NA) lens
The final resolution achievable by SRRF depends on multiple factors, including the strength of the fluctuations used to generate the correlations, the labelling density and the photo-stability of the fluorophores; in the best-case scenario it shows resolution approaching that of localisation based super-resolution methods while utilising a wider range of hardware and fluorophores
Summary
Adapting the 3D-printed Openflexure microscope enables computational super-resolution imaging [version 1; peer review: 2 approved].
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