Adaptations in lipid metabolism in adipose tissue induced by high intensity training

Abstract

This study investigated the effects of high intensity training on metabolic regulation of lipid metabolism. Male Wistar were randomized to the controlor training group. Progressive tests of running speed were performed in order to determine the maximal velocity (Vmax) and total time exercise (TTF). On five consecutive days, during ten weeks, the rats performed 60 min of exercise at 75%Vmax. PPARα and CPT1 mRNA expression were determined by qRT-PCR. AMPKp, ACC, ATGL and CD36 protein quantities were determined by western blotting. Intramyocellular triacylglycerol (IMTG) was extracted. Citrate synthase activity (CSA) was analyzed. Data were analyzed: unpaired Student's t-test (P<0.05). The training protocol increased TTF on the trained group and this response was followed by reduction on adiposity index, without changes in body weight. The trained group showed reduction in IMTG and increase in CSA in muscle tissue. It was observed increase in ATGL and CD36 in muscle and adipose tissues and no changes in PPARα and AMPKp, however CPT1 was reduced in adipose tissue, while, it was increased in muscle tissue in trained rats. ACC in adipose tissue was increased in trained rats, but in muscle tissue it was not different. The high intensity training promotes changes in lipid metabolism in adipose tissue and these responses could improve the energy supply to active muscle. Supported: CNPq