Abstract
Recent discussion1, 23 of the size and nature of influenza virus has been based upon the properties of strains which have been adapted to the extra-embryonic fluids of the chick embryo.This note is presented in the belief that it is important for other viruses to be adapted to a similar technic so as to be available for similar study.The strain of epidemic keratoconjunctivitis virus4 used in this investigation, was isolated by tissue culture and established in mouse brain by Major M. Sanders, MC, AUS. The original material received from Major Sanders was given 2 mouse brain passages, and approximately 10% suspensions of whole macerated brain from the last passage were inoculated into the chorio-allantoic fluid of 8-day chick embryos. The embryos died in 5 or 6 days, the chorio-allantoic and amniotic fluids were collected, tested for sterility, and pooled.After 2 more embryo-fluid passages, the fluid from the third passage was reinoculated into young mice. The mouse brain material was again passed three ti...
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