Abstract
Traditional microscopy provides only for a small set of magnifications using a finite set of microscope objectives. Here, a novel architecture is proposed for quantitative phase microscopy that requires only a simple adaptation of the traditional off-axis digital holographic microscope. The architecture has the key advantage of continuously variable magnification, resolution, and Field-of-View, by simply moving the sample. The method is based on combining the principles of traditional off-axis digital holographic microscopy and Gabor microscopy, which uses a diverging spherical wavefield for magnification. We present a proof-of-concept implementation and ray-tracing is used to model the magnification, Numerical Aperture, and Field-of-View as a function of sample position. Experimental results are presented using a micro-lens array and shortcomings of the method are highlighted for future work; in particular, the problem of aberration is highlighted, which results from imaging far from the focal plane of the infinity corrected microscope objective.
Highlights
IntroductionPublisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations
For the system proposed here, a microscope objective (MO) replaces the camera, which we model as a thin lens in the same plane as the camera
The condenser is expected to produce an ideal diverging spherical wavefront at the sample plane, which will in practice contain aberrations which will contribute to aberration in the image
Summary
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Holography [1,2] is an imaging methodology that involves separate processes for recording and replay in order to recover the image. Photographic films were required to record the holograms and the reconstruction process was implemented optically; in the past two decades this approach has been superseded by the application of a digital area sensor to record the holograms, and the reconstruction process is performed using a set of computer algorithms that simulate optical replay [3,4]. Several architectures exist for optically recording a digital hologram. The off-axis technique, initially developed for the case of photographic film [2], enables separation of the noisy
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