Abstract
Routine cell culture demands the use of animal-derived products, mainly fetal bovine serumand swineor bovine trypsin. According to the 3Rs principle and to theEuropean Centre for the Validation of Alternative Methods, animal-free substitutes are strongly recommended for in vitro methods. In this study, theHEp-2 cell line was adapted to different totally animal-free culture systems, such as a serum-free complete medium (VP-SFM), human platelet lysate and a synthetic trypsin (TrypLE™ Express); afterward, cell growth was assessed with the xCELLigence instrument. Animal-free products provided promising results, with performances similar or preferable to the common reagents; thereforetheir use could be encouraged for bothethical and technical advantages.
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