Abstract
Antispermal antibodies were detected by flow cytofluorimetry and mixed antiglobulin reaction (MAR). The results of the two methods significantly correlated regarding serum IgG antispermal antibodies under conditions ruling out capacitation of spermatozoa. There were no significant differences in the content of antispermal antibodies in the sera of fertile, pregnant women, women with endometriosis and adhesions, or in the peritoneal fluid of fertile and sterile women. In women with endometriosis and adhesions the level of antispermal antibodies in the peritoneal fluid was significantly higher than in the serum. The use of flow cytofluorimetry markedly improved the detection of antispermal antibodies in sterile women.
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