Abstract
Summary The objective of this study was to compare an indi rect ELISA, based on a purified 60 kDa envelope glycoprotein (gp51SU), with a Pourquire indirect ELISA for the detection of antibodies to the bovine leukemia virus. For conducting this research, 340 s erum samples were collected from two different bree ds of cows (Sarabi and Holestin) in different herds. Commercial ELISA revealed positive results in 17 (7%) Holstein cows. An appropriate ELISA cut-off was determined by receiver operating curve (ROC) analysis in comparison with commercial indirect ELISA. Results showed a relative sensitivity and specificity of 97 % and 92%, respectively, for a cut-off value of 0.34 in the domestic ELISA. In conclusion, the results o f the present study showed that domestic developed kit ca n be used for diagnosis of bovine leukemia virus wi th appropriate sensitivity and specificity. In additio n, a comparison of the results from a native breed, Sarabi, with Holstein showed that there was no significant (P>0.05) difference in the frequency of infection w ith BLV between the two breeds.
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