ADAMTS-3’ ün (A Disintegrin and Matrix Metalloproteinase Type,1 Motif 3) Normal ve Kanserli Hücre Hatlarındaki Ekspresyon Paterni

Abstract

ADAMTS-3 is a procollagen N- proteinase whose main function is to cleave procollagen II, the principal collagen of the cartilage and bone. This maturation process provides accurate fibril conformation for tissues. Along with collagen II, ADAMTS-3 can also process some other extracellular matrix components such as collagen III and fibronectin. ADAMTS mediated processing of the extracellular matrix components has considerable importance in both normal and pathological circumstances. Therefore, substrate specificities and the tissue expression profiles of the family members have become a hot topic. Further, altered expressions of the family members have been elucidated in some tumor types. Therefore, protumor and /or tumor suppressor functions of them is being investigated. The expression profile of the ADAMTS-3 has been studied only in a few normal and cancer tissues. Knowledge of the expression patterns of the ADAMTS-3 in cell lines are rather limited. In the present study, we determined ADAMTS-3 mRNA (sqRT-PCR) and protein expression levels (western-blot) in normal HUVEC, and cancer cell lines, namely, Saos-2, MG-63, DU-145, PC-3, Hep3B and PANC-1 cells. ADAMTS-3 was highly expressed in HUVEC, Saos-2, MG-63, and PC-3 cells. These findings will be fundamental for future studies that will be performed on ADAMTS-3 transcriptional regulation studies specific cancer types and also defining of the ADAMTS-3 contribution on the development and metastasis of specific cancer types.