ADAMTS-18 in the host tissues exerts little effect on breast tumor progress in a murine 4T1 breast cancer model

Min Liu,Fang Jing,Wei Zhang,Suying Dang,Tiantian Lu

doi:10.1186/s12952-016-0045-0

Min Liu, Fang Jing + Show 3 more

Open Access

https://doi.org/10.1186/s12952-016-0045-0

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Abstract

BackgroundIn this study, we aimed to identify a novel extracellular proteinase ADAMTS-18 that could be a potential tumor suppressor gene.ResultsWe successfully constructed Adamts-18 knockout mice with BALB / c background. RT-PCR analysis showed syngeneic mammary tumor cell line 4 T1 per se has weakly endogenous ADAMTS-18 expression. Orthotopic inoculation of 4 T1 cells within the mammary fat pad of host mice, we found no significant difference in tumor growth and metastasis between Adamts-18 knockout mice and widetype control.ConclusionsWe did not confirm that ADAMTS-18 in the host tissues is relevant for breast tumor progress in a murine 4 T1 breast cancer model.

Highlights

In this study, we aimed to identify a novel extracellular proteinase ADAMTS-18 that could be a potential tumor suppressor gene
Since ADAMTS-18 is one of these genes located around 16q23 region, it has been studied as a candidate oncogene [3, 5,6,7,8,9,10]
Because ADAMTS-18 is often inactivated in breast tumors, we develop Adamts-18 knockout mouse with BALB/c background, and inoculate syngeneic mammary tumor cell line 4 T1 cells to evaluate the role of ADAMTS-18 in breast cancer

Summary

Introduction

We aimed to identify a novel extracellular proteinase ADAMTS-18 that could be a potential tumor suppressor gene. RT-PCR analysis showed syngeneic mammary tumor cell line 4 T1 per se has weakly endogenous ADAMTS-18 expression. Orthotopic inoculation of 4 T1 cells within the mammary fat pad of host mice, we found no significant difference in tumor growth and metastasis between Adamts-18 knockout mice and widetype control. Matrix metalloproteinases are traditionally linked to tumor dissemination through their ability to degrade most extracellular matrix (ECM) components, facilitating tumor cell invasion and metastasis [1]. It has been shown that ADAMTS-18 gene was inactivation in many carcinomas especially breast tumors and, is regarded as a novel functional tumor suppressor [3]. To further study the role of ADAMTS-18 in vivo, we generated Adamts-18

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