Acylase I catalysed hydrolysis of para-substituted( S)-phenylalanine derivatives from mixtures of the racemic ortho- and para-substituted isomers

Abstract

para-Substituted( S)-phenylalanines may be obtained by treatment of the corresponding mixtures of ortho- and para-substituted N-acetyl-( RS)-phenylalanines with Acylase I from porcine kidney. The selectivity of the enzyme may be attributed to its evolution to digest peptide derivatives of ( S)-phenylalanine and ( S)-tyrosine.