Abstract
Diopatra claparedii which is colloquially known as Ruat Sarung can be found along the west coast of Peninsular Malaysia. The species has a unique ability to regenerate anterior and posterior segments upon self-amputation or injury, thus having potential as a wound healing promoter. In this study, the wound healing potential of D. claparedii aqueous extract on acute wound model in rats was revealed for the first time. Various concentrations (0.1%, 0.5%, and 1.0% w/w) of D. claparedii ointment were formulated and tested on Sprague Dawley rats through topical application on full-thickness skin wounds for 14 days. The wound healing effects were investigated via behaviour observation, wound contraction, and histopathological analysis. Quality assessment was performed via skin irritation test, microbial contamination test (MCT), and heavy metal detection. The study also included test for antibacterial activities and detection of bioactive compounds in D. claparedii. One percent of D. claparedii ointment showed rapid wound healing potential with good soothing effects and more collagen deposition in comparison to the commercial wound healing ointments such as acriflavine (0.1% w/v) and traditional ointment gamat (sea cucumber extract) (15.0% w/v). No local skin irritation, microbial contamination, and insignificant concentration of heavy metals were observed, which indicate its safe application. Moreover, the aqueous extract of D. claparedii exhibited antibacterial activities against Escherichia coli and Pseudomonas aeruginosa with minimum inhibitory concentration (MIC) value at 0.4 g/ml. 1H NMR analysis of the aqueous extract of D. claparedii revealed some metabolites that might be responsible for its wound healing properties such as amino acids, halogenated aromatics, organic acids, vitamins, and others. Altogether, these results suggested that the aqueous extract of D. claparedii could be utilised as an alternative natural wound healing promoter.
Highlights
Skin is the human body’s largest organ, representing 15% of total adult body weight, and acting as mechanical barriers to external environment
Animal Behaviour. e behaviour of Sprague Dawley rats towards the treatment was observed after the trauma was inflicted and continued as wound healing progressed on days 3, 7, 11, and 14. ree criteria were monitored including withdrawal pain response, sleeping/resting, and cage exploration
Results from this study showed the levels of arsenic, cadmium, and lead were not detected, which means most of the heavy metal compositions were less than 0.1 mg/kg, except for mercury (Table 4). us, it suggested that polychaete ointment (PO) is safe to be used as the level of tested heavy metals did not exceed the maximum limit set by Malaysia's National as the prepared stock solution of extract (0.4 g/ml) did not Pharmaceutical Regulatory Agency (NPRA) guideline
Summary
Skin is the human body’s largest organ, representing 15% of total adult body weight, and acting as mechanical barriers to external environment. A proper wound management including effective treatment is essential to achieve complete wound healing process that would halt progression of acute wound to chronic wound. Is species is found widely at mudflat in mangrove or estuary area along the west coast of Peninsular Malaysia [10] It has a segmented body with parapodia that bear chaetae. Us, they can be found by bait diggers when low tides and sold for fishing purposes It has a unique feature where it can regenerate both anterior and posterior segment upon injury or self-amputation [12]. Due to this distinct feature, it has been hypothesised to have wound healing potential, yet no reports were available until today. In phylum Annelida, many polychaetes were reported to possess significant medical applications such as anti-cancer, anti-inflammatory, and antimicrobial agents, but there is still less information about polychaete application in wound healing treatment [13]. erefore, the study intends to discover the wound healing potential and chemical properties of the D. claparedii aqueous extract using full-thickness wound in animal model
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Evidence-based complementary and alternative medicine : eCAM
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
