Acute Toxicity Study of Intravenously Administered Capecitabine Resealed Erythrocytes in Mice

Abstract

Introduction:- Capecitabine resealed erythrocytes were prepared as a potential targeted drug delivery system for the treatment of hepatocellular carcinoma. This system has been developed to increase the therapeutic efficacy of drug and to reduce its adverse effects. This paper demonstrated the acute toxicity study of the formulation in Swiss albino mice. Methodology:- Dose of Capecitabine was 2000 mg/kg orally with zero toxicity as per the previous studies. The doses of formulation selected were 1/5th, 1/10th, 1/20th of the oral dose calculated on the basis of the % encapsulation efficiency of drug in formulation. The doses were given intravenously in single dose in mice tail vein. All the animals were observed for 14 days period for mortality, behavioral symptoms and locomotor activity. Results:- After sacrification of animals, serum was collected to analyze hematological and biochemical parameters. The isolated liver was observed for histopathological changes in mice. Here, we found that all the three to be selected would be safe for further in- vivo screening of Hepatocellular carcinoma. Conclusion:- So, we concluded that, all the three doses of formulation low (0.90mg/kg), medium (1.79mg/kg) and high (3.58mg/kg) with no signs of mortality, behavioural changes and no necrosis in liver histopathology. This revealed that, all the three selected doses were safe for further in vivo studies.