Acute toxicity of palm oil mill effluent on zebrafish (Danio rerio Hamilton-Buchanan, 1822): Growth performance, behavioral responses and histopathological lesions

Abstract

Evaluating the toxicity of Palm Oil Mill Effluent (POME) is critical as part of the effort to develop waste management regulations for the palm oil industry. In this study, we investigated the acute toxicity of POME on growth performance, behavioral response, and histopathology of gill and liver tissues of zebrafish (Danio rerio). In total, 550 adult male zebrafish were used for the toxicity experiment including range finding test, acute toxicity test, growth performance and behaviour test. Static non-renewal acute toxicity bioassays were conducted by exposing fish to POME (1.584–9.968 mL/L) for 96 h. Growth performance, behavior response, and histopathological lesions in untreated and POME treated (96-h LC50: 5.156 mL/L) fish were measured at 24, 48, 72 and 96 h. Time-dependent significant decline in body length and body weight of POME-exposed zebrafish was observed. Furthermore, several behavioral changes were recorded, including hyperactivity, loss of balance, excessive mucus secretion, and depigmentation. Decreasing operculum movement and oxygen consumption rate as well as alterations in gill tissues (i.e. hyperplasia, hypertrophy, hemorrhage, and necrosis) of POME-exposed zebrafish were observed, suggesting a dysfunction in respiratory performance. On the other hand, liver tissue alterations (congestion, hemorrhage, hyperplasia, shrinkage of hepatocytes, hydrophilic degeneration, and necrosis) indicated a disruption in detoxification performance. We conclude that exposure to POME at acute concentration caused histopathological lesions both in gill and liver tissue along with changes in fish behaviors which disrupted respiratory and detoxification performance, resulting in mortality and reduced growth of zebrafish. These findings might provide valuable information for guiding POME management and regulation.