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Abstract
Penaeus monodon juveniles (total length 28.4±6.2 mm) were exposed to different concentrations of nitrate–N (nitrate as nitrogen) using the static renewal method at different salinity levels. The 48-, 72-, 96-h LC50s (median lethal concentrations) of nitrate–N for P. monodon juveniles were 2876, 1723, 1449 mg/l in seawater of 15‰ (g/kg); 3894, 2506, 1575 mg/l in 25‰; 4970, 3525, 2316 mg/l in 35‰, respectively. The “safe level” for rearing juvenile P. monodon was estimated to be 145, 158 and 232 mg/l nitrate–N in 15‰, 25‰ and 35‰, respectively. The relationship between LC50 of nitrate–N (mg/l), salinity ( S), exposure time ( t) and the interaction between salinity and exposure time (St) is as follows: LC50=3917.467+102.456S−42.634t−0.342 St (R 2=0.775)
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