Abstract
Four-day static renewal acute toxicity test was used for Heteropneustes fossilis to determine the LC 50 values for chromium chloride. Six replicates each containing ten fish (kept in glass aquarium containing 30 L of the test solution) were subjected to chromium chloride (50, 75, 100, 125, 150, 200 and 250 mg/L). The per cent mortality after exposure to various concentrations of chromium chloride to H. fossilis for 24, 48, 72 and 96 h has been observed and the LC 50 values at different exposure periods as well as their upper and lower confidence limits and slope functions were calculated by PoloPlus software version 2.0 Computer programme. The LC50 values of chromium chloride for H. fossilis at 24, 48, 72, and 96 h were calculated as 138.50 mg/L; 119.38 mg/L; 92.77 mg/L and 63.26 mg/L, respectively.
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