Abstract
This study investigated the acute toxicity of different concentrations of arsenic trioxide (As2O3; ATO) on rat lungs. In total, 160 Wistar rats were randomly divided into the control, low‐, medium‐ and high‐dose groups, which were exposed to 0, 0.16, 1.60 and 16 μg/kg of ATO by intratracheal instillation, respectively. Samples were collected at 6, 12, 24, 48 and 72 hours after exposure and the dynamic changes indicative of acute lung toxicity were monitored. Compared with the control group, the exposure groups exhibited significant changes such as increased lung water content ratio and protein concentration in the bronchoalveolar lavage fluid, pulmonary interstitial thickening, cell membrane edema, increased inflammatory factor concentration, JNK and P38 were significantly activated, and the degree of phosphorylation was increased. Furthermore, all the changes in the exposure groups were exposure concentration‐dependent. ATO respiratory tract exposure can cause restrictive ventilatory disturbance in rats, and the degree of injury is exposure concentration‐dependent.
Highlights
This study investigated the acute toxicity of different concentrations of arsenic trioxide (As2O3; ATO) on rat lungs
The hematoxylin and eosin (H&E) staining illustrated in Figure 2A shows that compared with the CON group there was significant edema in the alveolar structure after ATO exposure
The H&E staining illustrated in Figure 2B shows that compared with the CON group, the exposure groups exhibited no obvious mucus exudation or occasional infiltration of inflammatory cells, but significant damage to the terminal bronchiole epithelia was observed using the optical microscope
Summary
0023373), weighing 100‐150 g, were purchased from the Experimental Animal Center of the Military Medical Science Academy of the Chinese People's Liberation Army. Each group was further randomly divided into five subgroups of eight rats each, which were sampled 6, 12, 24, 48 and 72 hours after exposure. 2.5 | Measurement of total protein concentration in bronchoalveolar lavage fluid. The bronchoalveolar lavage fluid (BALF) was recovered and placed in a 15 mL centrifuge tube, centrifuged at 352 g for 10 minutes at 4°C, the supernatant was collected, and the total protein concentration was determined using the Coomassie brilliant blue G250 method. Groups were compared using a one‐way analysis of variance and a P < .05 was considered statistically significant
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