Acute Toxicity of Ethyl Acetate Fraction of Kaempferia galanga L. Rhizome

Abstract

The Kaempferia galanga L. (KG) rhizome has a long history of being used as a variety of anti-inflammatory, analgesic, antioxidant, sedative, antibacterial, and anti-cancer compounds. The KG rhizomes have the potential to be an effective complementary medicine, but their use must be completely safe. This study examines the acute toxicity of the ethyl acetate fraction of KG (EAFKG) with parameters of blood chemistry value, and liver and kidney histological morphology. 40 DDY strain mice, consisting of males and females with weights ranging from 20-35 g, were used in this study. The mice are divided into four groups for each sex. Groups 1, 2, 3, and 4 received the EAFKG at a dose of 0.128; 0.64; 3.2; and 16 g/Kg BW, respectively, and were administered orally. Observations are carried out for 24 hours. The levels of serum creatinine, AST, and ALT were detected with a clinical spectrophotometer. According to the test results, both male and female mouse groups had no fatalities. The EAFKG rhizome is practically non-toxic (LD50 > 15 g/Kg BW). There is no significant difference in AST, ALT, and serum creatinine levels at any dose group than the control group. EAFKG administration affects liver and kidney cells at high doses but does not cause lethality.