Acute toxicity, bioaccumulation and elimination of prometryn in tilapia (Oreochromis niloticus)

Abstract

Tilapia juvenile (Oreochromis niloticus) (mean weight 50.00 ± 10.00 g) were aqueous exposed to different concentrations of the herbicide prometryn to investigate its acute toxicity, bioaccumulation and uptake and elimination rates. First, a 96-h acute toxicity test was carried out. The resulting 96 h LC50 was 5.49 mg/L, and the 96 h LC10 was 5.02 mg/L. Then, fish were exposed to 0.55 mg/L (1/10 96 h LC50) and 0.055 mg/L (1/100 96 h LC50) of prometryn solution for 28 days, followed by 14 days of elimination in clean groundwater. The result shows that in both water and tissues, prometryn concentrations fluctuated during the exposure period, indicating that steady state was not reached. The bioaccumulation of prometryn was the highest in liver, followed by gill, muscle and blood. The accumulated concentration levels in various tissues were always higher in the high concentration compared to the low concentration. The highest accumulated concentration of prometryn in various tissues in the 0.055 mg/L treatment were for muscle: 0.136 ± 0.0616 mg/kg (1 d), liver: 3.74 ± 2.95 mg/kg (7 d), gill: 0.971 ± 1.45 mg/kg (1 d) and blood: 0.0716 ± 0.0669 mg/kg (22 d). In the 0.55 mg/L treatment, the highest levels were for muscle: 1.27 ± 0.284 mg/kg (1 d), liver: 16.9 ± 12.7 mg/kg (7 d), gill: 8.11 ± 3.02 mg/kg (1 d) and blood: 0.751 ± 0.0775 mg/kg (22 d). The highest bioconcentration factor (BCF) of 93.1 was observed in the liver when exposed to the low concentration. Besides, for other tissues, the highest BCF were for muscle: 5.76, gill: 32.3 and blood: 2.91, all observed in the 0.55 mg/L treatment. Most of the accumulated prometryn was removed from all tissues within 24 h after the organisms were transferred to clean water. However, management of using prometryn in China aquaculture should be improved to prevent possible ecotoxicological effects and ensure food safety.