Acute Toxicity, Antioxidant, and Antifatigue Activities of Protein-Rich Extract from Oviductus ranae.

Yang Zhang,Yao Dong,Hongli Zhou,Liping Mao,Xiaoxiao Xu,Hao Cui,Yang Liu,Kun Zhu

doi:10.1155/2018/9021371

Abstract

The paper investigated the preparation, amino acid composition, acute toxicity, and in vitro and in vivo antioxidant, coupled with in vivo antifatigue activities of protein-rich extract of Oviductus ranae (PEOR). The results indicated that PEOR possesses high-safety property with maximum tolerated dose (MTD) higher than 20 g/kg in mice, shows weak scavenging capacities against hydroxyl, superoxide anion, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, as well as ferric-reducing antioxidant power in vitro, but exerts strong antioxidant effect in ethanol-induced oxidative stress mice model; it can decrease malonaldehyde (MDA) and protein carbonyl (PCO) formation and increase total superoxide dismutase (T-SOD) activity and glutathione (GSH) synthesis. Besides the strong in vivo antioxidant activity, PEOR in a dose of 400 mg/kg also has antifatigue effect in mice, and it can prolong the exhaustive swimming time, reduce the elevated blood urea nitrogen (BUN) and blood lactic acid (BLA) caused by intense exercise. The in vivo activity of PEOR may be contributed by its absorbed amino acids, due to the fact that eight antioxidant amino acids and twelve glucogenic ones were found in it. This study will provide an evidence for the clinical use of PEOR as a dietary supplement for antioxidant and antifatigue in the same oral dose (400 mg/kg).

Highlights

Some harmful factors including overconsumption of drinking and smoking, X-ray irradiation, organic pollutants, and heavy metals can cause the overproduction of reactive oxygen species (ROS), which subsequently destroy the dynamic equilibrium between ROS generation and elimination to induce oxidative stress [1]
Seventeen amino acids were noted in protein-rich extract of Oviductus ranae (PEOR), seven of them were essential amino acids, which accounted for 41.9%
In view of increasing number of therapeutic risks caused by the use of natural products [45,46,47] and our previous work [21], where we found that Oviductus ranae (OR) possesses high-safety property, in present study, only a single-dose oral toxicity with an observation of 14-day interval was conducted to evaluate the safety of PEOR, and 20 g/kg was taken as an upper limit dose

Summary

Introduction

Some harmful factors including overconsumption of drinking and smoking, X-ray irradiation, organic pollutants, and heavy metals can cause the overproduction of reactive oxygen species (ROS), which subsequently destroy the dynamic equilibrium between ROS generation and elimination to induce oxidative stress [1]. Due to the fact that levels or activities of endogenous antioxidants, such as glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalases (CAT), are always lower than the level demanded for free radical-scavenging, it is usually necessary to supplement exogenous antioxidant when facing oxidative stress [9]. Another critical role of antioxidant lies in its positive effects on chronic fatigue syndrome (CFS) [10]. An increasing number of papers have demonstrated that antioxidant exerts antifatigue activity in vivo, especially for some natural products [11,12,13,14].

Results

Discussion

Conclusion