Acute Toxicity, and Neurobehavioural Responses of Afidopyropen on Exposure to Freshwater Edible Fish, Cyprinus carpio (Linnaeus)

Abstract

Nowadays water pollution has become a real concerning problem worldwide in the aquatic ecosystem, due to the increasing use of a variety of pesticides in agriculture for the growing food demand in the world. Thereby in this investigation, we examined the LC50 value of afidopyropen, an insecticide, in addition to selected behavioural and ACh, AChE activity of Cyprinus carpio fingerlings. Fingerlings were exposed to different concentrations of afidopyropen ranging from 0.4mg to 1.6 mg/L by the semi-static bioassay method. And it had been found to be 1.0 mg/L. The fish exposed to this insecticide showed remarkable behavioural abnormalities in a dose-dependent manner. ACh accumulation and AChE inhibition in carp (Cyprinus carpio) exposed to a different concentration for the period of 96 hrs caused a significant increase in the elevation of acetylcholine (ACh), and was related to a marked decrease in the activity of acetylcholinesterase (AChE). In comparison with the corresponding controls, AChE activity was significantly decreased, whereas the ACh accumulation was markedly elevated due to the interaction of AChE activity which remarks in excess accumulation of acetylcholine at the neuromuscular junctions and synapses leading to make them more excitable for the behavioural changes due to impaired neurophysiology of the fish brain. The toxicity of afidopyropen during exposure to fish suggests that this compound poses a greater risk to fish.