Acute toxic effects of microcystin-LR on crayfish (Procambarus clarkii): Insights from antioxidant system, histopathology and intestinal flora.

Abstract

To study the toxic effects of microcystin-LR (MC-LR) on crayfish, adult male Procambarus clarkii were exposed to different concentrations of MC-LR for 96h. In the meantime, the accumulation characteristics of MC-LR and the alternations of antioxidant system, histopathology and intestinal flora of P. clarkii were investigated. The results demonstrated that the hepatopancreas, gills and intestines of P. clarkii could effectively accumulate MC-LR. Antioxidant-related genes such as Mn-sod, cat, gst, gpx, mt and hsp70 showed different expression trends in different organs to respond to MC-LR-induced oxidative stress. MC-LR led to histological changes in the hepatopancreas, gills and intestines, thus affecting their corresponding physiological functions. Additionally, the abundances of bacterial phyla including Firmicutes and Planctomycetes and genera including Dysgonomonas, Brevundimonas and Anaerorhabdus in the intestine were significantly changed after MC-LR exposure, and the disruption of intestinal flora might further cause abnormal intestinal microbial metabolism and genetics in P. clarkii. This study provides novel mechanistic insights into the toxic impacts of microcystins on aquatic crustaceans. HIGHLIGHTS: • MC-LR was significantly accumulated in the hepatopancreas, gills and intestines of P. clarkii. • MC-LR induced the differential expression of antioxidant-related genes of P. clarkii. • MC-LR caused histological alterations in the hepatopancreas, gills and intestines of P. clarkii. • MC-LR affected the intestinal microbial composition and function of P. clarkii.