Acute Social Defeat Stress Increases Sleep in Mice.

Shinya Fujii,Michael Lazarus,Xuzhao Zhou,Mahesh K Kaushik,Mustafa Korkutata

doi:10.3389/fnins.2019.00322

Shinya Fujii, Michael Lazarus + Show 3 more

Open Access

https://doi.org/10.3389/fnins.2019.00322

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Abstract

Social conflict is a major source of stress in humans. Animals also experience social conflicts and cope with them by stress responses that facilitate arousal and activate sympathetic and neuroendocrine systems. The effect of acute social defeat (SoD) stress on the sleep/wake behavior of mice has been reported in several models based on a resident-intruder paradigm. However, the post-SoD stress sleep/wake effects vary between the studies and the contribution of specific effects in response to SoD or non-specific effects of the SoD procedure (e.g., sleep deprivation) is not well established. In this study, we established a mouse model of acute SoD stress based on strong aggressive mouse behavior toward unfamiliar intruders. In our model, we prevented severe attacks of resident mice on submissive intruder mice to minimize behavioral variations during SoD. In response to SoD, slow-wave sleep (SWS) strongly increased during 9 h. Although some sleep changes after SoD stress can be attributed to non-specific effects of the SoD procedure, most of the SWS increase is likely a specific response to SoD. Slow-wave activity was only enhanced for a short period after SoD and dissipated long before the SWS returned to baseline. Moreover, SoD evoked a strong corticosterone response that may indicate a high stress level in the intruder mice after SoD. Our SoD model may be useful for studying the mechanisms and functions of sleep in response to social stress.

Highlights

All living organisms respond to any external biological source of stress, i.e., perceived or actual threats, with a predictable biological pattern in an attempt to restore the internal homeostasis of the body (Chrousos, 2009)
We found that slow-wave sleep (SWS) strongly increased in response to social defeat (SoD), whereas rapid eye movement sleep (REMS) was only moderately increased for a limited period several hours after the SoD session
We established a mouse model of sleep alterations after acute SoD stress based on a resident-intruder paradigm, whereby a C57BL/6j male mouse was introduced as an intruder to a resident CD-1 male mouse selected for its high level of aggression (Figure 1)

Summary

Introduction

All living organisms respond to any external biological source of stress, i.e., perceived or actual threats, with a predictable biological pattern in an attempt to restore the internal homeostasis of the body (Chrousos, 2009). Social conflicts are a major source of stress for humans and play a major role in the pathogenesis of affective disorders like anxiety and depression (Charney and Manji, 2004; Huhman, 2006). Social conflicts occur in animals (Toyoda, 2017). A social animal with the inability to dominate its opponent shows submissive behavior and accepts a lower social rank to avoid injury and death that could occur if the animal continues to act in an aggressive manner (Huhman, 2006). Rodent models of social defeat (SoD) stress are often based on a resident-intruder paradigm where the resident attempts to defend its home cage from the intruder who eventually subordinates itself to the unfamiliar territorial

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