Acute seizures in the acute ischemic stroke setting

Abstract

<h3>Abstract</h3> The chromosome of archetypal bacteria <i>E. coli</i> is riddled with multifaceted complexity and its multiscale organization is slowly getting recognised. The emergence of chromosome conformation capture techniques and super-resolution microscopy are providing newer ways to explore chromosome organisation, chromosome dynamics and its effect on gene expression. Here we combine a beads-on-a-spring polymer model and recently reported high-resolution Hi-C data of <i>E. coli</i> chromosome to develop a comprehensive model of <i>E. coli</i> chromosome at 5 kbp resolution. The Hi-C data-integrated chromosome model elucidates a self-organised structure of <i>E. coli</i> chromosome into multiple macrodomains within ring-like architecture, with oriC loci located at the mid-cell position. The model also predicts that a majority of the genetic loci are linearly organised, except Ter macrodomain. The distance profiles, extracted from the model are in quantitative agreement with data from FISH and DNA-recombination assay experiments. Additionally, a genome-wide fine-grained radius of gyration map captures multiple chromosomal interaction domains (CIDs) and identifies the location of rrn operons. Finally, via a mutation-based analysis, the model quantifies the role of multiple nucleotide-associated proteins (NAPs) like HU, Fis and MatP in controlling the chromosome architecture.