Abstract
The eruption of Mt. St. Helens on May 18, 1980, resulted in a concentrated short-term exposure for a large segment of the population of eastern Washington, northern Idaho, and western Montana. Samples were collected from all three regions and the respirable size particles (⋜0.6μ) were separated and analyzed. The predominance of the respirable size fraction was found by X-ray energy dispersive analysis to be alumino silicates. A homogeneous preparation was intratracheally injected into young adult guinea pigs. The dose given the animals consisted of 52.5 mg in saline. This dose provided a short-term heavy exposure setting similar to that of the populus. At the end of the selected post-injection intervals of either 2 or 4 hours or 1 through 7 days, the animals were sacrificed by vascular perfusion through the right ventricle.Embedded tissue samples were reviewed at the light microscopy level and selected fields were thin sectioned for transmission electron microscopy and scanning transmission electron microscopy (correlated with X-ray energy dispersive analysis). The EM was carried out on a JEOL 100CX TEMSCAN.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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