Abstract
BackgroundMeasuring the effects of the acute intake of natural products on human biomarker concentrations, such as those related to oxidation and inflammation, can be an advantageous strategy for early clinical research on an ingredient or product.Methods31 total healthy subjects were randomized in a double-blinded, placebo-controlled, acute pilot study with post-hoc subgroup analysis on 20 of the subjects. The study examined the effects of a single dose of a polyphenol-rich beverage (PRB), commercially marketed as "SoZo®", on serum anti-inflammatory and antioxidant markers. In addition, phytochemical analyses of PRB, and in vitro antioxidant capacity were also performed.ResultsAt 1 hour post-intake, serum values for 8-iso-PGF2-alpha and advanced oxidation protein products decreased significantly by 40% and 39%, respectively. Additionally, there was a trend toward decreased C-reactive protein, and increased nitric oxide levels. Both placebo and PRB treatment resulted in statistically significant increases in hydroxyl radical antioxidant capacity (HORAC) compared to baseline; PRB showed a higher percent change (55-75% versus 23-74% in placebo group), but the two groups did not differ significantly from each other.ConclusionsPRB produced statistically significant changes in several blood biomarkers related to antioxidant/anti-inflammatory effects. Future studies are justified to verify results and test for cumulative effects of repeated intakes of PRB. The study demonstrates the potential utility of acute biomarker measurements for evaluating antioxidant/anti-inflammatory effects of natural products.
Highlights
Inflammation is associated with and/or implicated in numerous disease states, such as metabolic syndrome, coronary artery disease, diabetes, erectile dysfunction, arthritis, obesity and cancer [1,2,3]
The present study examines the effect of short-term consumption of a polyphenol-rich beverage (PRB) on several biomarkers related to antioxidant/anti-inflammatory processes
Results were expressed as grams of gallic acid equivalents [31]. 2.2.3 Total anthocyanin content Total anthocyanin content was determined by UV-vis spectrophotometry, using malvidin 3-glucosinolate (PhytoLab, Vestenbergsgreuth, Germany) as an external standard. 2% methanolic hydrochloric acid (HCl) solution was used for anthocyanins extraction
Summary
Inflammation is associated with and/or implicated in numerous disease states, such as metabolic syndrome, coronary artery disease, diabetes, erectile dysfunction, arthritis, obesity and cancer [1,2,3]. While inflammation serves as a normal and necessary response to tissue injury and infections, excessive inflammation can be Intake of plant polyphenol-containing foods and products has been associated with beneficial levels of antioxidant/anti-inflammatory markers, and health promoting effects in epidemiological, in vitro, ex vivo and in vivo studies [4,5,6,7,8] It is not always clear whether direct, rapid antioxidant/anti-inflammatory effects, or longer-term regulatory actions are responsible. Weeks increases serum radical scavenging capacity based on a total antioxidant status assay; yet adding isoflavones in vitro to plasma pooled from subjects samples drawn prior to treatment had little effect [9] The latter lack of effect occurred even if isoflavones were added well in excess of what oral intake could produce in serum samples. Measuring the effects of the acute intake of natural products on human biomarker concentrations, such as those related to oxidation and inflammation, can be an advantageous strategy for early clinical research on an ingredient or product
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
