Abstract
Gamma oryzanol (GO) is well known for its antioxidant activity and health-promoting benefits. The gamma oryzanol-loaded zein nanoparticles (GOZNs) were successfully prepared in our previous study. In the present work, the acute oral toxicity of GOZNs was evaluated based on OECD guideline 420. GOZNs were fabricated by the liquid-liquid dispersion and lyophilized. The samples displayed a mean diameter of 311.20±3.01 nm and high loading capacity of 311.22±7.97 to 322.69±5.67 mg-GO/g-powder after 42-day storage at -18oC. Healthy female Sprague Dawley rats (8 weeks of age) were used for the experiments. Five female rats were administered a single dose of 2,000 mg GO/kg body weight via the oral route. Observations of toxicity signs were recorded for the first 24 hours, and the changes in the general physical conditions were monitored for 14 days before the gross necropsy on day 15. The results show that Sprague Dawley rats exhibited normal growth, and neither mortality nor acute toxicity signs were observed throughout the study period. The findings revealed that GOZNs did not have acute toxicity and were safe when administered orally in Sprague Dawley rats for short periods with LD50 > 2,000 mg/kg.
Highlights
Rice is the most widely consumed staple food in Asia, especially in Thailand, where the rice production in 2020 was 18.6 million tonnes [1]
The present study aimed to evaluate the acute oral toxicity of gamma oryzanol-loaded zein nanoparticles (GOZNs) in Sprague Dawley rats according to OECD No 420 [26]
The GOZNs prepared in this study exhibited a nanoscale diameter, low polydispersity index, and highly negative zeta-potential, as well as the good retention of Gamma oryzanol (GO) during 42-day storage at -18oC
Summary
Rice is the most widely consumed staple food in Asia, especially in Thailand, where the rice production in 2020 was 18.6 million tonnes [1]. The evaluation of in vivo toxicity of GOZNs is considered essential for their application in food products. The interactions between each compound in the consumed products and the biological systems could lead to adverse effects, requiring the assessments of the immune response, metabolism patterns, biodistribution, and obliteration of nanoparticles [20]. Pan and Zhong [21] suggested that applications of nanoparticles require in vitro and in vivo studies to guide the development of safe food products that utilize the unique functionalities of nanoparticles. Toxicological studies in animals were raised to identify the possibility of adverse effects from the accumulation of nanoparticles in the body on the immune system, oxidative stress-related disorders, and other diseases [22,23]. The present study aimed to evaluate the acute oral toxicity of GOZNs in Sprague Dawley rats according to OECD No 420 [26]. Toxicity signs of each rat were recorded for the first day, and changes in general physical conditions were investigated for days before examining internal organs by gross necropsy on day
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
