Abstract
Acute megakaryoblastic leukemia is an uncommonly recognized disorder, usually diagnosed by using electron microscopy to detect platelet peroxidase or with monoclonal antibody to localize the factor VIII related antigen. We described a 41-year-old female patient with acute megakaryoblastic leukemia identified by localizing the factor VIII related antigen in megakaryoblastic with immunoperoxidase technique. Clinically she presented symptoms of anemia and markedly elevated platelet count. 47% unclassified agranular blasts were seen on a bone marrow asiration smear. Bone marrow biopsy showed diffuse proliferation of blasts, micromegakaryocytes and atypical megakaryocytes. The cytochemical staining, included periodic-acid shiff (PAS), Sudan-Black B (SBB), peroxidase, non-specific esterase (NSE) and naphthol ASD chloroacetate (NASD), all appeared negative on the blast cells. Using monoclonal antibody (DAKO, anti-factor VIII), various stages of maturation in megakaryocytic series were identified. She was treated with low-dose Ara-C, but died due to an infection in a pancytopenic state ten days after chemotherapy.
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