Acute immobilization stress alters LH and ACTH release in response to administration of N-methyl-D,L-aspartic acid in peripubertal and adult male rats.

Abstract

We have evaluated the effects of stress on the pituitary hormone response to N-methyl-D,L aspartic acid (NMA) in peripubertal (43 day old) and adult (80 day old) male rats. Animals were stressed by immobilization for 3 h. Fifteen min prior to the end of the stress period, animals were injected sc with either vehicle or increasing doses of NMA (10, 30 or 60 mg/kg BW). Animals were sacrificed 15 minutes later. A significant rise in plasma LH in response to NMA occurred at a lower dose (10 versus 60 mg/kg BW), and the magnitude of the response was greater overall in peripubertal than in adult unstressed rats. Stress altered the LH response to NMA in both age groups. In peripubertal stressed rats, NMA did not induce a rise in plasma LH levels. In adult rats exposed to stress, the magnitude of the LH response to 60 mg/kg BW of NMA was lower than in unstressed animals. NMA administration triggered a rise in plasma ACTH levels in unstressed rats of both age groups, but the magnitude of the rise was greater in adults than in peripubertal animals. Stress reduced the ACTH response to NMA administration in both age groups. Plasma FSH concentrations were higher in peripubertal animals than in adults. NMA and stress were without major effect on plasma FSH levels in either age group. These data suggest that excitatory amino acids (EAA) form an important component in the regulatory processes governing the release of LH and ACTH, and that the maturational stage of the central nervous system and exposure to stress alter the LH and ACTH response to EAA.