Abstract
An outbreak of acute hemorrhagic conjunctivitis occurred in Delhi, India, during August and September 1996. The etiologic agent was confirmed as enterovirus type 70 by a modified centrifugation-enhanced culture method followed by immunofluorescence and neutralization tests. After nearly a decade, this virus is reemerging as a cause of acute hemorrhagic conjunctivitis in India.
Highlights
An outbreak of acute hemorrhagic conjunctivitis occurred in Delhi, India, during August and September 1996
Cover-slip monolayers infected with a known enterovirus 70 (EV-70) prototype (Kono)like strain from a previous outbreak (5) and 10 of the 13 clinical specimens showed specific cytoplasmic fluorescence with monoclonal antibodies to EV-70
A new enterovirus (EV-70) was identified as the etiologic agent of acute hemorrhagic conjunctivitis (AHC) (7); subsequently it spread to other parts of Africa and Asia including India
Summary
An outbreak of acute hemorrhagic conjunctivitis occurred in Delhi, India, during August and September 1996. We conducted a study to identify the etiologic agent by viral culture, immunofluorescence and neutralization tests, and polymerase chain reaction (PCR). Hep-2cell monolayers were grown in 24-well plates, containing 12-mm cover slips (Bellco Glass Inc., New Jersey, USA).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
