Acute effects of unilateral carotid artery occlusion on the cerebrocortical microcirculation in mice

Abstract

Event Abstract Back to Event Acute effects of unilateral carotid artery occlusion on the cerebrocortical microcirculation in mice András Iring1*, Béla Horváth1, Gábor Lenzsér2, Marcus Tillson3, Björn Bakken3 and Zoltán Benyó1 1 Semmelweis University, Institute of Human Physiology and Clinical Experimental Research, Hungary 2 University of Pécs, Department of Neurosurgery, Hungary 3 Perimed AB, Järfälla, Sweden Carotid artery occlusion in humans usually results in no or minimal neurological signs, indicating that the regulatory mechanisms of the cerebral circulation are able to adapt well to changes of the perfusion pressure in the circle of Willis. Our further goal is to identify the underlying mechanisms by investigating transgenic mice. As a first step we analyzed the effects of unilateral carotid artery occlusion on the cerebrocortical microcirculation of control C57BL/6J mice. Cerebrocortical blood flow (CoBF) was measured with laser-speckle technique in both hemispheres. After a stable baseline blood flow, the left common carotid artery was ligated, and after 10 minutes, the mice were exposed to 5 % CO2. CoBF was determined before and after the ligation and during hypercapnia. Low frequency (100-200 mHz) CoBF-oscillations were determined with Fourier analysis in order to evaluate, whether the vessels were dilated to their maximal degree, which is indicated reportedly by the disappearance of vasomotion. CoBF in the left hemisphere reduced by 10 %, while remained unchanged in the contralateral side. During hypercapnia, blood flow increased equally in both hemispheres (on the left side by 78±4 %, on the right by 74±5 %). Low-frequency CoBF-oscillations remained unaltered after carotid ligation. During hypercapnia, however, their amplitude decreased significantly in both hemispheres, while the dominant frequency remained unchanged. Our results indicate that after carotid occlusion ipsilateral CoBF diminished by a smaller degree then expected, which indicates the development of compensatory vasodilatation. However, since slow-wave oscillation remains unchanged, the relaxation has not reached its maximal extent. This conclusion is also supported by the fact that hypercapnia was able to enhance CoBF on the affected side and diminished the oscillations as well. Support: NKTH OMFB-00770/2009, TÁMOP 4.2.2. - 08/1/KMR-2008-004, OTKA K62375 and an EFSD/Servier Grant Award. Conference: IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010. Presentation Type: Poster Presentation Topic: Abstracts Citation: Iring A, Horváth B, Lenzsér G, Tillson M, Bakken B and Benyó Z (2010). Acute effects of unilateral carotid artery occlusion on the cerebrocortical microcirculation in mice. Front. Neurosci. Conference Abstract: IBRO International Workshop 2010. doi: 10.3389/conf.fnins.2010.10.00232 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 May 2010; Published Online: 04 May 2010. * Correspondence: András Iring, Semmelweis University, Institute of Human Physiology and Clinical Experimental Research, Budapest, Hungary, iringa@freemail.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers András Iring Béla Horváth Gábor Lenzsér Marcus Tillson Björn Bakken Zoltán Benyó Google András Iring Béla Horváth Gábor Lenzsér Marcus Tillson Björn Bakken Zoltán Benyó Google Scholar András Iring Béla Horváth Gábor Lenzsér Marcus Tillson Björn Bakken Zoltán Benyó PubMed András Iring Béla Horváth Gábor Lenzsér Marcus Tillson Björn Bakken Zoltán Benyó Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.