Acute contact with imidacloprid in soil affects the nesting and survival success of a solitary wild bee, Osmia lignaria (Hymenoptera: Megachilidae)

Abstract

We assessed impacts of direct acute contact with imidacloprid-treated soil on nesting behavior and mortality of the blue orchard mason bee (Osmia lignaria Say), which is a native solitary cavity-nesting species that collects mud for nest partitions. Laboratory-reared O. lignaria females were exposed to three concentrations of imidacloprid (0, 50, 390 and 780 ppb), in wet (30% moisture) soil for 20 min and released in large flight cages, where impacts on nesting activity and nest cell production were evaluated. Mortality was tested in another experiment using exposure at the same concentrations with two differing soil moisture levels (20% and 40%). Nesting activity was reduced by 42% for females exposed at 390 ppb and by 66% for females exposed at 780 ppb. Females treated at 780 ppb produced 40% fewer nest cells per day. Sex ratios of F1 generation were skewed toward male in the 50 ppb treatment group with 50% fewer females. The number of cells and pre-pupae per nest, as well as the weight of pre-pupal cocoons did not vary among exposure levels. There were no mortality effects at 20% soil moisture for any level of imidacloprid, but at 40%, mortality of females was >50% at all levels of imidacloprid. These results suggest that acute exposure to imidacloprid residue in soil can have negative impacts on soil-interacting bees, and the effects may be relative to the degree of soil moisture.