Acute and subchronic toxicological evaluation of Mequindox in Wistar rats

Abstract

We studied an acute and subchronic oral toxicity of Mequindox (MEQ), a quinoxaline 1,4-dioxide antimicrobial promoter, in Wistar rats according to OECD guidelines. For acute toxicity study, single doses of MEQ at 175, 550 and 2000mg/kg b.w. were administered to rats by oral gavage. The calculated LD50 was 550mg/kg b.w. In subchronic study, rats were fed diets containing 0, 55, 110 or 275mg MEQ/kg. There was a reduction in body weight of rats fed 275mg MEQ/kg diet. At 90days autopsy, a significant decrease in the kidney weight was observed in males while an increase in relative liver and adrenal weights were observed in females fed 275mg MEQ/kg diet. There was a significant increased in alanineaminotransferase (ALT) and malondialdehyde (MDA) concentrations in males, superoxide dismutase (SOD) activities in females, and aspartateaminotransferase (AST) levels in serum of both genders fed 275mg MEQ/kg diet. Other toxic effects of 275mg MEQ/kg diet included significant decrease in sodium and significant increase in potassium concentrations in serum in both genders. We may conclude that MEQ can induce hepatic and adrenal histological changes as well as leaking of different serum constituents in Wistar rats.