Acute and Subacute Toxicity Study of Lehana Yogas in Wistar Albino Rats

Abstract

Lehana Yogas is an Ayurvedic preparation which are known to enhance growth and development by providing sufficient nutrition and promoting health with improving intellect and speech and administered frequently with ghee and honey to children’s as immune boosters. In this study we assessed the safety parameters of the Lehana Yogas mentioned and its effect on acute and subacute administration in Wistar albino rats. The Wistar strain albino rats were divided into group of five, vehicle group of Ghrita-Madhu, Ghrita-Madhu with Suvarna Bhasma, Ghrita-Madhu -Suvarna Bhasma with Vacha Churna, Ghrita-Madhu -Suvarna Bhasma with Amalaki Churna, respectively. The duration of administration was for 28 days. During the course of study, no mortality and no significant behavioral changes were noticed in any group studied. No major alterations were observed in hematology and serum biochemistry reports. Histopathology of vital organs also shown no toxic effects. Materials and Methods: Thirty albino rats of either sex were selected and assigned to 5 groups of 6 each. Drugs will be administered for the duration of 28 days and will be assessed for toxicity profile at the end of the study. Result: Animals who were administered daily with Lehana Yogas for longer duration did not show any mortality. Hence, it indicates safer use of drugs in terms acute and subacute level of administration. Discussion: It is concluded that the administration of Lehana Yogas is safe and non-toxic at the tested dose levels.