Acute and sub-chronic toxicological evaluation of Boga -TN tablets in experimental animals

Abstract

Boga-TN is a formula originating from herbal medicines that are used to treat liver disorders; however, its toxicity is not yet investigated. Therefore, this study was conducted to assess the acute and sub-chronic toxicity of Boga-TN tablets in experimental animals. Acute toxicity study was performed via Swiss mice, with a single oral dose, and followed up to seven days according to World Health Organization Guidance. No sign of acute oral toxicity was detected, and the LD50 Boga-TN was estimated to be more than 60.38 g/kg. Based onobtained results , the sub-chronic toxicity study was carried out in Wistar rats for 4 consecutive weeks by oral administration at the doses of 0.77 and 2.31 g/kg/day. After treatment, no significant treatment-related abnormalities were observed at both doses of Boga-TN, compared to the control group, except lower neutrophil but higher lymphocyte values were observed in the treated animals. Histopathology assessment did not show any significant variation between control and treatment groups during the study period.