Acute Administration of Exogenous Lactate Increases Carbohydrate Metabolism during Exercise in Mice.

Inkwon Jang,Deunsol Hwang,Jisu Kim,Sunghwan Kyun,Kiwon Lim

doi:10.3390/metabo11080553

Abstract

In this study, we investigated the effects of exogenous lactate administration before exercise on energy substrate utilization during exercise. Mice were divided into exercise control (EX) and exercise with lactate intake (EXLA) groups; saline/lactate was administered 30 min before exercise. Respiratory gas was measured during moderate intensity treadmill exercise (30 min). Immediately after exercise, blood, liver, and skeletal muscle samples were collected and mRNA levels of energy metabolism-related and metabolic factors were analyzed. At 16–30 min of exercise, the respiratory exchange ratio (p = 0.045) and carbohydrate oxidation level (p = 0.014) were significantly higher in the EXLA than in the EX group. Immediately after exercise, the muscle and liver glycogen content and blood glucose level of the EXLA group were lower than those of the EX group. In addition, muscle mRNA levels of HK2 (hexokinase 2; p = 0.009), a carbohydrate oxidation-related factor, were higher in the EXLA than in the EX group, whereas the expression of PDK4 (pyruvate dehydrogenase kinase 4; p = 0.001), CS (citrate synthase; p = 0.045), and CD36 (cluster of differentiation 36; p = 0.002), factors related to oxidative metabolism, was higher in the EX than in the EXLA group. These results suggest that lactate can be used in various research fields to promote carbohydrate metabolism.

Highlights

There was no significant difference in metabolism during the total exercise period
To determine how the administration of lactate before exercise affects the use of energy substrates during exercise, we examined the expression of energy metabolismrelated mRNAs in the gastrocnemius skeletal muscle (Figure 7)
The purpose purpose of of this this study study was was to to investigate investigate the the effect effect of of exogenous exogenous lactate lactate adminadminThe istration as an exercise supplement on the use of energy substrates during exercise

Summary

Introduction

Supplement consumption before exercise is an important factor that directly affects metabolism during exercise, and many studies have been conducted on this topic [1,2] Consumption of supplements, such as capsaicin [3], caffeine [4], and carnitine [2,5], is known to improve muscular endurance performance by increasing the energy supply through fat oxidation during exercise [2,3,4,5]. The use of supplements is associated with side effects, such as excessive sensitivity and stomachache [4,5,6]. It is necessary to discover new exercise supplements with minimal side effects

Objectives

Methods

Results

Conclusion