Abstract
PURPOSE: Explore how acupuncture activates Ca2+ signaling and mitochondrial network homeostasis through TRP channels in eccentric exercise-induced skeletal muscle injury. The aim is reveal the mechanism of acupuncture which reduces scar production in injured skeletal muscles and promotes repair of skeletal muscle injuries. METHODS: 72 8-week-old C57BL6 mice were randomly divided into control group (C), electrical stimulation group (E), electrical stimulation and acupuncture group (EA), electrical stimulation and acupuncture group, electrical stimulation and acupuncture TRP, NCX (sodium-calcium exchanger) Ryanodine receptors (RYRs) inhibitor group (EAT), (EAN) and (EAR), transfect TA muscle with cDNAs encoding for either 2mtYC2 or pMitoTimer using electroporation, and mouse TA muscle injury model was established after 10 days of recovery, using stainless steel acupuncture needle for EA, EAT and EAN group. Imaging was performed on a Zeiss microscope Average YFP/CFP ratio values were determined and analyzed with ImageJ. Data analysis was made using two-way ANOVA with repeated measures. RESULTS: There were no statistical differences in the indicators of each group before the experiment (P > 0.05). The Ca2+ YFP/CFP ratio of group E was not significantly different from that of groups EAT, EAN, and EAR (P > 0.05), but it is significantly higher than the groups C, EA (1.50 + 0.20vs 0.80 + 0.20vs 1.00 + 0.20, P < 0.05). The area of mitochondrial damage in group E was not significantly different from that in groups EAT, EAN and EAR (P > 0.05), but it is larger than groups C, EA (2643.41 + 150.00 vs 0.00 + 10 vs 1452.26 + 150.00um2, P < 0.05); Outside the injury area, group E mitophagy area diameter is longer than group EA (164.56 + 30.92 vs 5.86 + 3.10 um + 0.20 P < 0.05) CONCLUSION: Acupuncture triggered TRP channel activating RyRs and NCX, prevented Ca2+ concentration from overloading, regulated intracellular mitochondrial Ca2+ uptake to maintain eccentric injury skeletal muscle mitochondrial network homeostasis.
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