Activity test of Guazuma ulmifolia Lam (Jati Belanda) methanol extract against bacteria Staphylococcus aureus and Klebsiella pneumoniae

Abstract

Background: Staphylococcus aureus and Klebsiella pneumoniae bacteria are a group of bacteria that can infect the respiratory tract so that they can cause complications for humans, Jati belanda leaves (Guazuma ulmifolia Lam) is a plant that has potential as an antibacterial, because it has active substances that can stop the growth of bacterial organisms, namely flavonoids, saponins, tannins, and alkaloids are its components. Purpose: This study aims to determine the antibacterial activity of methanol extract of Jati belanda leaves (Guazuma ulmifolia Lam) on the growth of Staphylococcus aureus and Klebsiella pneumoniae bacteria. Methods: Antibacterial activity test in this study was carried out using the well method, Staphylococcus aureus bacteria and Klebsiella pneumoniae bacteria as test microbes in this study, positive control (chloramphenicol), 20% DMSO negative control and five concentration groups namely 100%, 50%, 25%, 12.5% ​​and 6.25% from methanol extract of Jati belanda leaves (Guazuma ulmifolia Lam). Results: From the research results it is known that the methanol extract of Jati belanda leaves (Guazuma ulmifolia Lam) is able to inhibit Staphylococcus aureus bacteria with each inhibition zone at 100% concentration of 12.31 mm, 50% concentration of 10.48 mm, 25% concentration of 7.62 mm and the positive control (chloramphenicol) was 33.24 mm and the Klebsiella pneumonia bacteria at 100% concentration was 14.27 mm, 50% concentration was 11.14 mm and the positive control (chloramphenicol) was 40.6 mm.