Activity test of chitosan haruan (Channa striata) fish scales as antibiofilm agent againts biofilm of Porphyromonas gingivalis

Abstract

Background: Porphyromonas gingivalis has been known as the major bacterial that induce periodontal inflammation through biofilm formation. Biofilms contain important virulence factors such as lipopolysaccharides. These virulence factors can make it escape from the periodontal immune system and modulate biofilms by increasing tolerance to antibiofilm agents so that biofilm formation becomes uncontrolled. The Chitosan from Haruan (Channa Striata) fish scales contains a degree of deacetylation of 85.25% which is associated with the content of an amine group (-NH2) which is positively charged in the Chitosan haruan fish scale which can act as an antibiofilm. Objective: The aim of this study was to analyze the activity of Chitosan Haruan fish scales (Channa striata) as an antibiofilm agent on Porphyromonas gingivalis biofilms. Methods: The research design used in this study was a true experimental design with a post-test with a control group design using 4 treatments and 1 positive control. All treatments were carried out biofilm testing to obtain Optical Density. Results: The results showed that chitosan Haruan fish scales with a concentration of 2.5%, 10%, 20%, 40% proved to be able to damage the Porphyromonas gingivalis biofilm. Conclusion: Chitosan Haruan fish scales have the ability as an antibiofilm agent against P.gingivalis biofilms.